查看狗狗原发性心脏血管肉瘤的流行病学、临床和病理特征：51例的回顾的源代码

=== 尸检、组织病理学和免疫组化 (Necropsy, histopathology and immunohistochemistry) ===

对所有 51 只狗进行了完全尸检（单独尸检 34 例;肿瘤切除和尸检 17 例）。在尸检时，对切除的心脏进行大体检查，并确定任何肿瘤的确切位置。然后将其置于 10% 磷酸盐缓冲福尔马林中至少 5 天。固定后，在未进行肿瘤切除的34只狗中，将占位性病灶整体切除并称重，计算肿瘤重量与体重比（TW/BW）（即肿瘤重量（g/体重kg）。对于其余 17 只接受肿瘤切除的狗，当时已经对切除的肿块进行了称重。在每种情况下，从心脏肿块中取出至少 5 个组织块并通过常规方法处理。切片（5μm）用苏木精和伊红（HE）染色，用于光学显微镜检查。此外，还收集了所有内脏器官的组织样本，重点关注那些有肿瘤病变提示转移的器官。对于免疫组织化学研究，使用针对细胞蛋白波形蛋白（1：200，单克隆，克隆MNF 16; Dako，Glostrup，丹麦），细胞角蛋白（1：100，单克隆，克隆Vim 3B4; Dako）、CD31（1：40，单克隆，克隆JC70A; Dako）和血管性血友病因子（因子VIII相关抗原）（1：50，单克隆，克隆F8/86;  Dako）。

Necropsy, histopathology and immunohistochemistry: Complete necropsy was performed on all 51 dogs (necropsy alone 34; tumor resection and necropsy 17). At necropsy, the removed heart was subjected to gross examination, and the exact location of any tumors was identified. It was then placed in 10% phosphate-buffered formalin for at least 5 days. After fixation, in the 34 dogs that had not undergone tumor resection, mass lesions were excised en bloc and weighed, and the tumor weight-to-body weight ratio (TW/BW) was calculated (i.e. tumor weight in g/body weight in kg). For the remaining 17 dogs that had undergone tumor resection, the resected masses had been weighed at that time. In each case, at least 5 tissue blocks were taken from the cardiac mass and processed by routine methods. Sections (5 µm) were stained with hematoxylin and eosin (HE) for light microscopy. In addition, tissue samples were collected from all internal organs, focusing on those with tumorous lesions indicative of metastasis. For the immunohistochemical study, sections were stained by the avidin-biotin-peroxidase method (Vectastain, Vector Laboratories, Burlingame, CA, U.S.A.) using antibodies against the cellular proteins vimentin (1:200, monoclonal, clone MNF 16; Dako, Glostrup, Denmark), cytokeratin (1:100, monoclonal, clone Vim 3B4; Dako), CD31 (1:40, monoclonal, clone JC70A; Dako) and von Willebrand factor (factor VIII-related antigen) (1:50, monoclonal, clone F8/86; Dako).

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